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Test ID: SPNEU Streptococcus pneumoniae Antigen, Random, Urine

Reporting Name

Streptococcus pneumoniae Ag, U

Useful For

Rapid diagnosis of pneumococcal pneumonia

Clinical Information

Streptococcus pneumoniae is the most frequently encountered bacterial agent of community-acquired pneumonia (CAP). Because of the significant morbidity and mortality associated with pneumococcal pneumonia, septicemia, and meningitis, it is important to have diagnostic test methods available that can provide a rapid diagnosis. In instances where empirical antibiotics are provided for CAP without culture confirmation of S pneumoniae, antigen testing may be useful.


A positive result is indicative of pneumococcal pneumonia.



Negative for pneumococcal pneumonia, suggesting no current or recent pneumococcal infection. Infection due to Streptococcus pneumoniae cannot be ruled out since the antigen present in the sample may be below the detection limit of the test.

Report Available

Same day/1 day

Day(s) Performed

Monday through Friday

Clinical Reference

1. Plouffe JF, Moore SK, Davis R, Facklam RR: Serotypes of Streptococcus pneumoniae blood culture isolates from adults in Franklin County, Ohio. J Clin Microbiol. 1994 Jun;32(6):1606-1607

2. Johnston RB Jr: Pathogenesis of pneumococcal pneumonia. Rev Infect Dis. 1991 May-Jun;13 Suppl 6:S509-S517. doi: 10.1093/clinids/13.supplement_6.s509

3. Janoff EN, Musher DM: Streptococcus pneumoniae. In Bennett JE, Dolin R, Blaser MJ, eds. Mandell, Douglas, and Bennett's Principles and Practice of Infectious Diseases. 9th ed. Elsevier; 2020:2473-2491

Method Name

Immunochromatographic Membrane Assay

Specimen Type


Ordering Guidance

Pneumococcal pneumonia is best diagnosed by sputum culture. For more information, see SPUTS / Bacterial Culture, Aerobic, Respiratory with Antimicrobial Susceptibilities, Varies.

Specimen Required

Supplies: Urine Tubes, 10 mL (T068)

Container/Tube: Plastic, 10-mL urine tube

Specimen Volume: 2 mL

Collection Instructions:

1. Collect a random urine specimen.

2. No preservative.

3. Excessively bloody or very turbid specimens containing protein, cells, or particulates will be canceled as they can inhibit the function of the test.

4. Centrifuging to remove particulates is not approved.

5. Specimens with any dyes or unnatural color are not acceptable and will be canceled.

Specimen Minimum Volume

0.25 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Urine Refrigerated (preferred) 14 days
  Frozen  14 days
  Ambient  24 hours

Reference Values


Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information


LOINC Code Information

Test ID Test Order Name Order LOINC Value
SPNEU Streptococcus pneumoniae Ag, U 77949-6


Result ID Test Result Name Result LOINC Value
83150 Streptococcus pneumoniae Ag, U 77949-6


If not ordering electronically, complete, print, and send Infectious Disease Serology Test Request (T916) with the specimen.

Mayo Clinic Laboratories | Microbiology and Infectious Disease Catalog Additional Information: