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Test ID: MTRNA Trichomonas vaginalis, Nucleic Acid Amplification, Varies

Useful For

Detection of Trichomonas vaginalis in urine and male patient specimens

Reporting Name

T.vaginalis, Misc, Amplified RNA

Specimen Type

Varies


Necessary Information


Specimen source is required.



Specimen Required


Submit only 1 of the following specimens:

 

Specimen Type: Urine

Supplies: Aptima Urine Transport Tube (T582)

Container/Tube: Aptima Urine Specimen Transport Tube

Specimen Volume: 15 to 20 mL

Collection Instructions:

1. Patient should not have urinated for at least 1 hour prior to specimen collection.

2. Patient should collect first portion of random voided urine (first part of stream) into a sterile, plastic, preservative-free container.

3. Transfer 2 mL of urine into the Aptima urine specimen transport tube using the disposable pipette provided within 24 hours of collection. The correct volume of urine has been added when the fluid level is between the black fill lines on the Aptima urine transport tube.

 

Specimen Type: Urine (following prostatic massage)

Supplies: Aptima Urine Transport Tube (T582)

Container/Tube: Aptima Urine Specimen Transport Tube

Specimen Volume: 15 to 20 mL

Collection Instructions:

1. Patient should not have urinated for at least 1 hour prior to specimen collection.

2. Patient should void a small amount of urine prior to prostatic massage. Pre-massage urine can be discarded or submitted for other testing as applicable.

3. Patient then ceases voiding and a prostatic massage is performed by the urologist or other health care professional.

4. Collect post-massage urine into a sterile, plastic, preservative-free container.

5. Transfer 2 mL of post-massage urine specimen into the Aptima urine specimen transport tube using the disposable pipette provided within 24 hours of collection. The correct volume of urine has been added when the fluid level is between the black fill lines on the Aptima urine transport tube.

 

Specimen Type: Urethral

Supplies: Swab, Aptima Male/Female Collection (T583)

Container/Tube: Aptima Collection Unisex Swab

Specimen Volume: Swab

Collection Instructions:

1. Urethral specimens must be collected using an Aptima Collection Unisex Swab.

2. Patient should not have urinated for at least 1 hour prior to collection.

3. With a rotating movement, insert swab (blue shaft) 2 to 4 cm into urethra.

4. Once inserted, rotate swab gently at least 1 full rotation using sufficient pressure to ensure swab comes into contact with all urethral surfaces. Allow swab to remain inserted for 2 to 3 seconds.

5. Place swab in the Aptima transport tube provided in collection kit. Snap off swab at score line so swab fits into closed tube.

6. Cap tube securely and label tube with patient's entire name and collection date and time.


Specimen Minimum Volume

See Specimen Required

Specimen Stability Information

Specimen Type Temperature Time Special Container
Varies Refrigerated (preferred) 30 days APTIMA VIAL
  Ambient  30 days APTIMA VIAL

Clinical Information

Trichomonas vaginalis (TV) is a protozoan parasite that commonly infects the genital tract of men and women. It is now considered to be the most common curable sexually transmitted infection (STI) agent, with an estimated 3.7 million infected individuals in the United States.(1-4) Although up to 70% of infected individuals are asymptomatic, infections may be associated with vaginitis, urethritis, and cervicitis in women, and urethritis and prostatitis in men.(3) Patients that are infected with T vaginalis have an increased risk of acquiring other sexually transmitted infections such as HIV, while infections in pregnant women are associated with premature labor, low birth-weight offspring, premature rupture of membranes, and post-hysterectomy/post-abortion infection.(3)

 

Symptoms of T vaginalis overlap considerably with other sexually transmitted infections; therefore, laboratory diagnosis is required for definitive diagnosis. The most commonly used method for detection is microscopic examination of a wet-mount preparation of vaginal secretions. However, this method has only 35% to 80% sensitivity compared with culture.(5) Culture also suffers from relatively low sensitivity (38%-82%) when compared to molecular methods.(5) Culture is technically challenging and takes 5 to 7 days to complete. Molecular methods, such as the Aptima T vaginalis assay, offer high sensitivity and specificity for detection of trichomoniasis. The Aptima test utilizes target capture, transcription-mediated amplification (TMA), and hybridization protection assay (HPA) technologies for detection of T vaginalis ribosomal RNA (rRNA).

Reference Values

Negative

Interpretation

A positive result is considered indicative of current or recent Trichomonas vaginalis infection (trichomoniasis).

Clinical Reference

1. Weinstock H, Berman S, Cates W Jr: Sexually transmitted diseases among American youth: incidence and prevalence estimates, 2000. Perspect Sex Reprod Health. 2004 Jan-Feb;36(1):6-10

2. Soper D: Trichomoniasis: under control or undercontrolled? Am J Obstet Gynecol. 2004 Jan;190(1):281-290

3. Division of STD Prevention, National Center for HIV/AIDS, Viral Hepatitis, STD, and TB Prevention: Trichomoniasis-CDC Fact Sheet. Centers for Disease Control and Prevention (CDC); Reviewed February 27, 2020. Accessed September 10, 2020. Available at www.cdc.gov/std/trichomonas/stdfact-trichomoniasis.htm

4. Schwebke JR, Burgess D: Trichomoniasis. Clin Microbiol Rev. 2004 Oct;17(4):794-803

5. Wendel KA, Erbelding EJ, Gaydos CA, Rompalo AM: Trichomonas vaginalis polymerase chain reaction compared with standard diagnostic and therapeutic protocols for detection and treatment of vaginal trichomoniasis. Clin Infect Dis. 2002 Sep;35(5):576-580

Day(s) Performed

Monday through Saturday

Report Available

1 to 3 days

Test Classification

This test has been modified from the manufacturer's instructions. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

87661

LOINC Code Information

Test ID Test Order Name Order LOINC Value
MTRNA T.vaginalis, Misc, Amplified RNA 46154-1

 

Result ID Test Result Name Result LOINC Value
SRC6 SOURCE: 31208-2
35034 T.vaginalis, Misc, amplified RNA 46154-1

Method Name

Transcription Mediated Amplification

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.

Mayo Clinic Laboratories | Microbiology and Infectious Disease Catalog Additional Information:

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