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Test ID: MPRP Mycoplasmoides pneumoniae, Molecular Detection, PCR, Varies

Reporting Name

Mycoplasma pneumoniae PCR

Useful For

Diagnosis of infections due to Mycoplasmoides pneumoniae

Clinical Information

Mycoplasmoides pneumoniae, previously Mycoplasma pneumoniae, is a small bacterium transmitted via organism-containing droplets. It is a cause of upper respiratory infection, pharyngitis, and tracheobronchitis, particularly in children, and has been associated with approximately 20% of cases of community acquired pneumonia.(1) Central nervous system and cardiac manifestations are probably the most frequent extrapulmonary complications of infections due to M pneumoniae. The disease is usually self-limited although severe disease has been reported in immunocompromised patients.(2)


Identification of M pneumoniae by culture-based methods is time consuming and insensitive. Serology based assays for M pneumoniae have several drawbacks. The development of IgM antibodies takes approximately 1 week and the IgM response in adults may be variable or it may be decreased in immunosuppressed individuals.(3,4) Confirmation of the disease may be dependent on the observation of a 4-fold rise in IgG antibody titers between acute and convalescent specimens, several weeks following the initial onset of illness, providing clinical utility only for retrospective testing.(4) Real-time polymerase chain reaction testing offers a rapid and sensitive option for detection of M pneumoniae DNA from clinical specimens.


A positive result indicates the presence of Mycoplasmoides pneumoniae.


A negative result does not rule out the presence of M pneumoniae and may be due to the presence of inhibitors within the specimen matrix, or the presence of organisms at numbers below the limits of detection of the assay.

Report Available

3 to 4 days

Day(s) Performed

Monday through Sunday

Method Name

Rapid Polymerase Chain Reaction (PCR) using Light Cycler and Fluorescent Resonance Energy Transfer (FRET)

Specimen Type


Necessary Information

Specimen source is required.

Specimen Required

The high sensitivity of amplification by polymerase chain reaction requires the specimen to be processed in an environment in which contamination of the specimen by Mycoplasmoides pneumoniae DNA is unlikely.


Submit only 1 of the following specimens:


Specimen Type: Respiratory


-Bartels FlexTrans VTM-3 mL (T892)

-Jiangsu VTM-3 mL (T891)

-M4-RT (T605)

Sources: Bronchial washing, bronchoalveolar lavage, tracheal secretions, sputum


Preferred: Sterile container

Acceptable: Specimen in M4, M4-RT, M5, M6, or universal transport medium

Specimen Volume: 1 mL


Specimen Type: Swab


-Culturette (BBL Culture Swab) (T092)

-BD Eswab (T853)

-Steriflock NP Swab (T861)

-Nasopharyngeal Swab (Rayon Mini-Tip Swab) (T515)

-Bartels FlexTrans VTM-3 mL (T892)

-Jiangsu VTM-3 mL (T891)

-M4-RT (T605)

Sources: Throat, nasal, or nasopharyngeal


Preferred: Culture swab transport system (Dacron or rayon swab with aluminum or plastic shaft with either Stuart or Amies liquid medium)

Acceptable: Culture transport swab (Stuart's media) or place swab in M4, M4-RT, M5, M6, universal transport media, or ESwab

Specimen Volume: Swab

Collection Instructions:

1. Collect specimen by swabbing back and forth over mucosa surface to maximize recovery of cells.

2. Place swab back into swab cylinder.


Specimen Type: Fluid

Sources: Pleural, pericardial, cerebrospinal

Container/Tube: Sterile vial

Specimen Volume: 0.5 mL

Specimen Minimum Volume

Respiratory, Fluid: 0.5 mL
Swab: 1 swab

Specimen Stability Information

Specimen Type Temperature Time Special Container
Varies Refrigerated (preferred) 7 days
  Frozen  7 days

Reference Values

Not applicable

Test Classification

This test was developed, and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information


LOINC Code Information

Test ID Test Order Name Order LOINC Value
MPRP Mycoplasma pneumoniae PCR 29257-3


Result ID Test Result Name Result LOINC Value
SRCMP Specimen source 31208-2
62394 Mycoplasma pneumoniae PCR 29257-3


If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.

Mayo Clinic Laboratories | Microbiology and Infectious Disease Catalog Additional Information: