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HelpTest ID: LCADP Adenovirus, Molecular Detection, PCR, Plasma
Reporting Name
Adenovirus PCR, PUseful For
Aiding in diagnosing adenovirus infections using plasma specimens
Clinical Information
Human adenoviruses cause a variety of diseases, including pneumonia, cystitis, conjunctivitis, diarrhea, hepatitis, myocarditis, and encephalitis. In humans, adenoviruses have been recovered from almost every organ system. Infections can occur at any time of the year and in all age groups. Currently, there are over 50 adenovirus serotypes that have been grouped into 6 separate subgenera.
Although adenovirus can be recovered in cell culture, it can take up to 3 weeks for the virus to be identified by culture methods (Mayo's shell vial culture provides more rapid results, reported at 2 and 5 days). Polymerase chain reaction assays offer a rapid, specific, and sensitive means of diagnosis by detecting adenovirus DNA.
Interpretation
A positive result indicates the presence of adenovirus nucleic acid in the clinical specimen.
A negative result does not rule out the presence of adenoviruses because viral DNA may be present at levels below the detection limits of this assay.
Report Available
2 to 5 daysDay(s) Performed
Monday, Wednesday, Friday
Clinical Reference
1. Florescu DF, Schaenman JM: Adenovirus in solid organ transplant recipients: Guidelines from the American Society of Transplantation Infectious Diseases Community of Practice. Clin Transplant. 2019 Sep;33(9):e13527
2. Buckwalter SP, Teo R, Espy MJ, Sloan LM, Smith TF, Pritt BS: Real-time qualitative PCR for 57 human adenovirus types from multiple specimen sources. J Clin Microbiol. 2012 Mar;50(3):766-771. doi:10.1128/jcm.05629-11
3. Ebner K, Pinsker W, Lion T: Comparative sequence analysis of the hexon gene in the entire spectrum of human adenovirus serotypes: phylogenetic, taxonomic, and clinical implications. J Virol. 2005 Oct;79(20):12635-12642
4. Ebner K, Suda M, Watzinger F, Lion T: Molecular detection and quantitative analysis of the entire spectrum of human adenoviruses by a two-reaction real-time PCR assay. J Clin Microbiol. 2005 Jul;43(7):3049-3053
5. Jothikumar N, Cromeans TL, Hill VR, Lu X, Sobsey MD, Erdman DD: Quantitative real-time PCR assays for the detection of human adenoviruses and identification of serotypes 40 and 41. Appl Environ Microbiol. 2005 Jun;71(6):3131-3136
6. Robinson C, Echavarria M: Adenovirus. In: Murray PR, Baron EJ, Jorgensen JH, eds. Manual of Clinical Microbiology. ASM Press; 2007:1589-1600
7. Kaneko H, Maruko I, Iida T, et al: The possibility of human adenovirus detection from the conjunctiva in asymptomatic cases during a nosocomial infection. Cornea 2008 Jun;27(5):527-530
Method Name
Real-Time Polymerase Chain Reaction (PCR)/DNA Probe Hybridization
Specimen Type
Plasma EDTASpecimen Required
Collection Container/Tube: Lavender top (EDTA)
Submission Container/Tube: Screw-capped, sterile container
Specimen Volume: 1 mL
Collection Instructions: Centrifuge and aliquot plasma into a sterile, plastic vial.
Specimen Minimum Volume
0.3 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Plasma EDTA | Refrigerated (preferred) | 7 days | |
| Frozen | 7 days |
Reference Values
Negative
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
87798
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| LCADP | Adenovirus PCR, P | 21055-9 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 56088 | Adenovirus PCR, P | 21055-9 |
Forms
If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.
mml-respiratoryinfections, mml-giinfections