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HelpTest ID: IDENT Organism Referred for Identification, Aerobic Bacteria
Infectious
Reporting Name
Organism Refer for ID, Aerobic BactUseful For
Identification of pure isolates of aerobic bacteria
Differentiation of members of the Staphylococcus aureus complex (S aureus, Staphylococcus argenteus, Staphylococcus schweitzeri)
Clinical Information
Organisms are referred to confirm identification or when the identity is unknown. This may provide helpful information regarding the significance of the organism, its role in the disease process, and its possible origin.
Techniques employed may include conventional biochemical analysis, commercial identification strips or panels, matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry or sequencing nucleic acid of the 16S ribosomal RNA gene.
Interpretation
Genus and species are reported on aerobic bacterial isolates, whenever possible.
Bacillus species will be reported out as "Large spore-forming aerobic gram-positive Bacillus, not Bacillus cereus or Bacillus anthracis," unless species identification is specifically requested on the request form.
Report Available
5 to 10 daysDay(s) Performed
Monday through Friday
Clinical Reference
1. Carroll KC, Pfaller MA, eds. Manual of Clinical Microbiology. 12th ed. ASM Press; 2019
2. Procop GW, Church DL, Hall GD, et al, eds. Koneman's Color Atlas and Textbook of Diagnostic Microbiology. 7th ed. Lippincott Williams and Wilkins; 2017
Method Name
Dependent on organism submitted, 1 or more of the following methods will be used: Conventional Biochemical Testing, Commercial Identification Strips or Panels, Matrix-Assisted Laser Desorption/Ionization Time-of-Flight (MALDI-TOF) Mass Spectrometry, and 16S RNA Gene Sequencing
Specimen Type
VariesOrdering Guidance
Mayo Clinic Laboratories will not perform identification testing on suspected select agents (eg, Bacillus anthracis, Brucella species, Burkholderia mallei, Burkholderia pseudomallei, Francisella tularensis, and Yersinia pestis). Consult with your state health department or the Centers for Disease Control and Prevention regarding identification confirmation or exclusion of such isolates. For more information see www.selectagents.gov/sat/list.htm.
If susceptibility testing is needed, also order ZMMLS / Antimicrobial Susceptibility, Aerobic Bacteria, Varies. If susceptibilities are not appropriate and will not be performed, ZMMLS will be canceled at report time.
Additional Testing Requirements
If susceptibility testing is needed; also order ZMMLS / Antimicrobial Susceptibility, Aerobic Bacteria, Varies. If susceptibilities are not appropriate and will not be performed, ZMMLS will be canceled at report time.
Shipping Instructions
1. See Infectious Specimen Shipping Guidelines for shipping information.
2. Place specimen in a large infectious container and label as an etiologic agent/infectious substance, if appropriate.
Necessary Information
1. Specimen source is required.
2. Isolate description is required including: Gram stain reaction, morphology, and tests performed.
Specimen Required
Supplies: Infectious Container, Large (T146)
Specimen Type: Pure culture of organism from source cultured
Container/Tube: Agar slant or other appropriate media
Specimen Volume: Entire specimen
Collection Instructions:
1. Perform isolation of infecting bacteria.
2. Bacterial organism must be submitted in pure culture, actively growing. Do not submit mixed cultures.
Specimen Minimum Volume
See Specimen Required
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Varies | Ambient (preferred) | |
| Refrigerated | ||
Reference Values
Identification of organism
Test Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
87077-Organism Referred for Identification, Aerobic Bacteria
87077-Identification Commercial Kit (if appropriate)
87077-Ident by MALDI-TOF mass spec (if appropriate)
87077-Bacteria Identification (if appropriate)
87153-Aerobe Ident by Sequencing (if appropriate)
87077-Additional Identification Procedure (if appropriate)
87147 x 3-Serologic Agglut Method 1 Ident (if appropriate)
87147-Serologic Agglut Method 2 Ident (if appropriate)
87147 x 4-Serologic Agglut Method 3 Ident (if appropriate)
87147 x 2-6 - Serologic Agglut Method 4 Ident (if appropriate)
87077-Identification Staphylococcus (if appropriate)
87077-Identification Streptococcus (if appropriate)
87798-Identification by PCR (if appropriate)
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| IDENT | Organism Refer for ID, Aerobic Bact | 32367-5 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| IDENT | Organism Refer for ID, Aerobic Bact | In Process |
Testing Algorithm
When this test is ordered, the reflex tests may be performed at an additional charge. All aerobically growing bacteria submitted will be identified and billed, as appropriate.
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| COMM | Identification Commercial Kit | No, (Bill Only) | No |
| RMALD | Ident by MALDI-TOF mass spec | No, (Bill Only) | No |
| GID | Bacteria Identification | No, (Bill Only) | No |
| ISAE | Aerobe Ident by Sequencing | No, (Bill Only) | No |
| REFID | Additional Identification Procedure | No, (Bill Only) | No |
| SALS | Serologic Agglut Method 1 Ident | No, (Bill Only) | No |
| EC | Serologic Agglut Method 2 Ident | No, (Bill Only) | No |
| SHIG | Serologic Agglut Method 3 Ident | No, (Bill Only) | No |
| STAP | Identification Staphylococcus | No, (Bill Only) | No |
| STRP | Identification Streptococcus | No, (Bill Only) | No |
| SIDC | Ident Serologic Agglut Method 4 | No, (Bill Only) | No |
| PCRID | Identification by PCR | No, (Bill Only) | No |
Special Instructions
Forms
If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.
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