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Test ID: FGEN Fungal Culture, Routine

Reporting Name

Fungal Culture, Routine

Useful For

Diagnosing fungal infections from specimens other than blood, skin, hair, nails, and vagina (separate tests are available for these specimen sites)

Clinical Information

Many fungi in the environment cause disease in immunocompromised human hosts. Accordingly, the range of potential pathogenic fungi has increased as the number of immunosuppressed individuals (eg, persons with AIDS, patients receiving chemotherapy or transplant rejection therapy) has increased. Isolation and identification of the infecting fungus in the clinical laboratory can help guide patient care.


Positive cultures of yeast and filamentous fungi are reported with the organism identification.


The clinician must determine whether or not the presence of an organism is significant. A final negative report is issued after 24 days of incubation.

Report Available

24 to 35 days

Day(s) Performed

Monday through Sunday

Clinical Reference

Ashbee HR: General approaches for direction detection and identification of fungi. In: Carroll KC, Pfaller MA, Landry ML, et al, eds. Manual of Clinical Microbiology. 12th ed. Vol 1. ASM Press; 2019:2035-2055

Method Name

Conventional Agar Culture/Macroscopy/Microscopy/D2 rDNA Gene Sequencing/Real-Time Polymerase Chain Reaction (rtPCR)/Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF MS)

Dimorphic Pathogen Identification Confirmation: D2 rDNA Gene Sequencing/rtPCR/MALDI-TOF MS

Specimen Type


Ordering Guidance

Nocardia and the other aerobic actinomycetes are not fungi, therefore  a fungal culture should not be ordered. However, these organisms grow well on mycobacterial medium. When infection with this group of organisms is suspected, order CTB / Mycobacteria and Nocardia Culture, Varies.

Shipping Instructions

Specimen should arrive within 24 hours of collection.

Necessary Information

Specimen source is required.

Specimen Required

Specimen Type: Body fluid

Container/Tube: Sterile container

Specimen Volume: Entire collection


Specimen Type: Bone marrow

Container/Tube: Sterile container, SPS/Isolator system, or green top (lithium or sodium heparin)

Specimen Volume: Entire collection


Specimen Type: Fresh tissue

Container/Tube: Sterile container

Specimen Volume: Pea sized

Collection Instructions: Tissue should be placed in small amount of sterile saline or sterile water.


Specimen Type: Respiratory specimen

Container/Tube: Sterile container

Specimen Volume: Entire collection


Specimen Type: Swab

Fresh tissue or body fluid is the preferred specimen type instead of a swab specimen.

Sources: Dermal, ear, mouth, ocular, throat, or wound

Container/Tube: Culture transport swab (noncharcoal) Culturette or ESwab

Specimen Volume: Swab

Collection Instructions:

1. Before collecting specimen, wipe away any excessive amount of secretion and discharge, if appropriate.

2. Obtain secretions or fluid from source with sterile swab.

3. If smear and culture are requested or both a bacterial culture and fungal culture are requested, collect a second swab to maximize test sensitivity.


Specimen Type: Urine

Container/Tube: Sterile container

Specimen Volume: 2 mL

Collection Instructions: Collect a random urine specimen.

Specimen Minimum Volume

Bone marrow or body fluid: 1 mL
Respiratory specimen: 1.5 mL
All other specimen types: See Specimen Required

Specimen Stability Information

Specimen Type Temperature Time
Varies Refrigerated (preferred) 7 days
  Ambient  7 days

Reference Values


If positive, fungus will be identified.

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

87102-Fungal culture, routine

87106-Id MALDI-TOF Mass Spec Yeast (if appropriate)

87107-Id MALDI-TOF Mass Spec Fungi (if appropriate)

87107-Fungal identification panel A (if appropriate)

87107-Fungal identification panel B (if appropriate)

87150 x 2-Identification Histoplasma/Blastomyces, PCR (if appropriate)

87153-D2 fungal sequencing identification (if appropriate)

87176-Tissue processing (if appropriate)

87150- Id, Candida auris Rapid PCR (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
FGEN Fungal Culture, Routine 580-1


Result ID Test Result Name Result LOINC Value
FGEN Fungal Culture, Routine 580-1

Testing Algorithm

When this test is ordered, the reflex tests may be performed at an additional charge.


For more information see Meningitis/Encephalitis Panel Algorithm.

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
D2F D2 Fungal Sequencing Identification No, (Bill Only) No
FUNA Fungal Ident Panel A No, (Bill Only) No
FUNB Fungal Ident Panel B No, (Bill Only) No
LCCI Ident Rapid PCR Coccidioides No, (Bill Only) No
LCHB Id, Histoplasma/Blastomyces PCR No, (Bill Only) No
RMALF Id MALDI-TOF Mass Spec Fungi No, (Bill Only) No
TISSR Tissue Processing No, (Bill Only) No
RMALY Id MALDI-TOF Mass Spec Yeast No, (Bill Only) No
LCCA Id, Candida auris Rapid PCR No, (Bill Only) No


If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.

Mayo Clinic Laboratories | Microbiology and Infectious Disease Catalog Additional Information:

mml-immunocompromised, mml-respiratoryinfections