Sign in →

Test ID: CTBID Culture Referred for Identification, Mycobacterium and Nocardia, Varies

Infectious

Reporting Name

Culture Refer for ID, Mycobacterium

Useful For

Rapid identification to the species level for Mycobacterium species, Nocardia species, and other aerobic actinomycete genera and species from pure culture isolates

Specimen Type

Varies


Shipping Instructions


1. See Infectious Specimen Shipping Guidelines in Special Instructions.

2. Place specimen in a large infectious container (T146) and label as an etiologic agent/infectious substance.



Necessary Information


1. Specimen source is required.

2. Isolate description is required: Gram stain reaction, morphology, tests performed.



Specimen Required


Specimen Type: Organism in pure culture

Supplies: Infectious Container, Large (T146)

Container/Tube: Middlebrook (7H10 or 7H11) or Lowenstein-Jensen medium slant or in broth (eg, Mycobacteria Growth Indicator Tube [7H9] broth)

Specimen Volume:

Visible growth of isolate on solid media

Isolate in broth media: ≥3 mL

A minimum volume of 3 mL is recommended in order to perform all initial testing, this may include: stains, sub-culture media, nucleic acid probes, and any additional testing that may be required to determine the identification. If the broth sample volume is <3 mL, initial testing may be limited, and increased turnaround time is likely.

Collection Instructions: Organism must be in pure culture, actively growing. Do not submit mixed cultures.


Specimen Stability Information

Specimen Type Temperature Time
Varies Ambient (preferred)
  Refrigerated 

Reference Values

Not applicable

Day(s) Performed

Monday through Sunday

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

LOINC Code Information

Test ID Test Order Name Order LOINC Value
CTBID Culture Refer for ID, Mycobacterium 543-9

 

Result ID Test Result Name Result LOINC Value
CTBID Culture Refer for ID, Mycobacterium 543-9

Testing Algorithm

When this test is ordered, the reflex test may be performed at an additional charge. All mycobacteria and Nocardia (including aerobic actinomycetes) submitted will be identified and billed as appropriate.

 

See Culture Referred for Identification Mycobacterium and Nocardia Algorithm.

Clinical Information

There are approximately 200 recognized species of mycobacteria and more than 100 Nocardia species. Many of these species are human pathogens and, therefore, identification to the species level is important to help guide patient care. In addition, there are other aerobic actinomycete genera that can be human pathogens including, but not limited to, Tsukamurella, Rhodococcus, and Gordonia species.

 

Nucleic acid hybridization probes are utilized that identify specific ribosomal RNA sequences of Mycobacterium tuberculosis complex, Mycobacterium avium complex, and Mycobacterium gordonae. Other Mycobacteria species, Nocardia species and other aerobic actinomycete genera are identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) or nucleic acid sequencing of a 500-base pair region of the 16S ribosomal RNA gene.

Interpretation

Organisms growing in pure culture are identified to the species level whenever possible.

Clinical Reference

1. Martin I, Pfyffer GE, Parrish N: Mycobacterium: General characteristics, laboratory detection and staining procedures. In: Carroll KC, Pfaller MA, Landry ML, et al, eds. Manual of Clinical Microbiology. 12th ed. Vol 1.  ASM Press; 2019:558-575

2. Warshauer DM, Salfinger M, Desmond E, Lin SYG: Mycobacterium tuberculosis complex. In: Carroll KC, Pfaller MA, Landry ML, et al, eds. Manual of Clinical Microbiology. 12th ed. Vol 1.  ASM Press; 2019:576-594

3. Caulfield AJ, Richter E, Brown-Elliott BA, Wallace RJ Jr, Wengenack NL: Mycobacterium: Laboratory characteristics of slowly growing mycobacteria other than Mycobacterium tuberculosis. In: Carroll KC, Pfaller MA, Landry ML, et al, eds. Manual of Clinical Microbiology. 12th ed. Vol 1. ASM Press; 2019:595-611

 4. Brown-Elliott BA, Wallace RJ Jr: Mycobacterium: Clinical and laboratory characteristics of rapidly growing mycobacteria. In: Carroll KC, Pfaller MA, Landry ML, et al, eds. Manual of Clinical Microbiology. 12th ed. Vol 1.  ASM Press; 2019:612-629

5. Conville PS, Brown-Elliott BA, Witebsky FG: Nocarida, Rhodococcus, Gordonia, Actinomadura, Streptomyces and other eerobic Actinomycetes. In: Carroll KC, Pfaller MA, Landry ML, et al, eds. Manual of Clinical Microbiology. 12th ed. Vol 1.  ASM Press; 2019:525-557

Report Available

60 to 70 days

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
RMALM Id MALDI-TOF Mass Spec AFB No, (Bill Only) No
RTBSP Id, Mtb Speciation, PCR No, (Bill Only) No
TBMP Mycobacteria Probe Ident No, (Bill Only) No
TBPB Mycobacteria Probe Ident Broth No, (Bill Only) No
ISMY ID by 16S Sequencing No, (Bill Only) No
LCTB Id, MTB complex Rapid PCR No, (Bill Only) No

CPT Code Information

Culture Referred for Identification, Mycobacterium

87118-Identification of mycobacteria

87158-Identification of mycobacteria by other methods (if appropriate)

87118 -Id MALDI-TOF Mass Spec AFB (if appropriate)

87153-Mycobacteria Identification by Sequencing (if appropriate)

87150-Mycobacteria Probe Ident, Solid (if appropriate)

87150-Mycobacteria Probe Ident, Broth (if appropriate)

87150-Id, Mtb Speciation, PCR (if appropriate)

87150- Id, MTB complex Rapid PCR (if appropriate)

Method Name

Nucleic Acid Probe/16S rDNA Sequencing/Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS)/Rapid PCR

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.

Mayo Clinic Laboratories | Microbiology and Infectious Disease Catalog Additional Information:

mml-mycobacteria