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Mayo Clinic Laboratories

Test ID: CTBBL Mycobacterial Culture, Blood

Reporting Name

Mycobacterial Culture, B

Useful For

Diagnosing mycobacteremia

Specimen Type

Whole blood


Shipping Instructions


Specimen must be processed within 7 days of collection.



Necessary Information


Specimen source is required.



Specimen Required


Container/Tube:

Preferred: Green top (sodium or lithium heparin)

Acceptable: SPS (sodium polyanethol sulfonate)

Specimen Volume: 8 to 10 mL per culture

Collection Instructions:

1. Send whole blood specimen in original tube.

2. SPS tubes are acceptable, but not preferred.

3. Note: SPS tubes must be clearly labeled as SPS. If label is obscured, sample may be canceled, as ACD (yellow top) is not an acceptable tube type.


Specimen Stability Information

Specimen Type Temperature Time
Whole blood Ambient (preferred) 7 days
  Refrigerated  7 days

Reference Values

Negative

If positive, mycobacteria are identified.

A final negative report will be issued after 42 days of incubation.

Day(s) Performed

Monday through Sunday

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

87116-Mycobacterial Culture

87118-Id MALDI-TOF Mass Spec AFB (if appropriate)

87150-Id, Mtb Speciation, PCR (if appropriate)

87150-Mycobacteria Probe Ident, Solid (if appropriate)

87153-Mtb PZA Confirmation, pcnA sequence (if appropriate)

87153-Mycobacteria Identification by Sequencing (if appropriate)

87150- Id, MTB complex Rapid PCR (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
CTBBL Mycobacterial Culture, B 64412-0

 

Result ID Test Result Name Result LOINC Value
CTBBL Mycobacterial Culture, B 64412-0

Clinical Information

Mycobacteremia occurs most often in immunocompromised hosts. The majority of disseminated mycobacterial infections are due to Mycobacterium avium complex but bacteremia can also be caused by other mycobacterial species including, but not limited to, Mycobacterium tuberculosis complex, Mycobacterium kansasii, Mycobacterium fortuitum, Mycobacterium chelonae, Mycobacterium scrofulaceum, Mycobacterium szulgai, and Mycobacterium xenopi.(1)

 

Mycobacterial blood cultures may be indicated for patients presenting with signs and symptoms of sepsis, especially fever of unknown origin.

Interpretation

A positive result may support the diagnosis of mycobacteremia.

Clinical Reference

1. Martin I, Pfyffer GE, Parrish N. Mycobacterium: General characteristics, laboratory detection, and staining procedures. In: Carroll KC, Pfaller MA, Landry ML, et al, eds. Manual of Clinical Microbiology. 12th ed. ASM Press; 2019:558-575

2. Crump JA, Morrissey AB, Ramadhani HO, et al. Controlled comparison of BacT/Alert MB system, manual myco/f lytic procedure, and isolator 10 system for diagnosis of mycobacterium tuberculosis bacteremia. J Clin Microbiol. 2011;49(8):3054-7. doi:10.1128/JCM.01035-11

3. Reimer LG. Laboratory detection of mycobacteremia. Clin Lab Med. 1994;14(1):99-105

Report Available

42 days

Specimen Minimum Volume

5 mL

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
ISMY ID by 16S Sequencing No, (Bill Only) No
RMALM Id MALDI-TOF Mass Spec AFB No, (Bill Only) No
RTBSP Id, Mtb Speciation, PCR No, (Bill Only) No
LCTB Id, MTB complex Rapid PCR No, (Bill Only) No

Testing Algorithm

When this test is ordered, the reflex tests may be performed at an additional charge.

Method Name

Continuously Monitored Automated Broth Culture Instrument with Conventional Methods for Identification of Mycobacteria

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.

Mayo Clinic Laboratories | Microbiology and Infectious Disease Catalog Additional Information:

mml-mycobacteria