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HelpTest ID: CHIKS Chikungunya Virus, PCR, Molecular Detection, Serum
Additional Testing Requirements
Due to the short period in which chikungunya RNA may be detected in serum, testing serum for IgM- and IgG-class antibodies to chikungunya virus is also recommended. See CHIKV / Chikungunya IgM and IgG, Antibody, Serum.
Testing for RNA or IgM-class antibodies to dengue and Zika viruses, concurrently with chikungunya virus testing should also be considered, given the overlapping clinical syndromes seen with these viruses.
Specimen Required
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container: Sterile container
Specimen Volume: 0.5 mL
Collection Instructions:
1. Within 2 hours of collection, centrifuge and aliquot the serum into a sterile container.
2. Serum specimens not aliquoted from the serum gel collection tube into a sterile container will be rejected.
Useful For
Qualitative detection of chikungunya virus in serum after early symptom onset (ideally <7 days)
This test is not recommended for screening healthy patients.
Method Name
Real-Time Reverse Transcription Polymerase Chain Reaction (PCR)/DNA Probe Hybridization
Reporting Name
Chikungunya Virus, PCR, SerumSpecimen Type
SerumSpecimen Minimum Volume
0.3 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Refrigerated (preferred) | 7 days | |
| Frozen | 7 days |
Clinical Information
Chikungunya virus (CHIK) is an RNA virus of the genus Alphavirus, family Togaviridae, transmitted mainly through the bite of infected mosquitoes in the genus Aedes (Aedes aegypti and Aedes albopictus). This is the same mosquito that transmits dengue, yellow fever, and Zika viruses. Most people infected with chikungunya virus will develop some symptoms, most commonly fever and joint pain. There is no specific antiviral treatment for chikungunya virus infection.
Most cases of disease have occurred in Africa, Asia, Europe, and the Indian and Pacific Oceans, but transmission of CHIK has been identified in Caribbean countries and South American regions, as well as foci in the southern United States. Infection with chikungunya virus may be suspected based on symptoms (fever, joint pain, and headache) and recent history of travel. A diagnosis of CHIK infection can be confirmed through laboratory tests on serum or cerebrospinal fluid.
This assay is designed to detect only species of clinical significance and is to be used for patients with a clinical history and symptoms consistent with chikungunya infection.
Reference Values
Negative
Reference values apply to all ages.
Interpretation
A positive test result indicates the presence of chikungunya virus RNA in the specimen.
A negative test result with a positive internal control indicates that chikungunya virus RNA is not detectable in the specimen.
A negative test result with a negative internal control is considered evidence of polymerase chain reaction inhibition or reagent failure. A new specimen should be collected for testing if clinically indicated.
Clinical Reference
1. Lanciotti RS, Kosoy OL, Laven JJ, et.al. Chikungunya virus in US travelers returning from India, 2006. Emerg Infect Dis. 2007;13(5):764-767
2. Johnson BW, Russell BJ, Goodman CH. Laboratory diagnosis of chikungunya virus infections and commercial sources for diagnostic assays. J Infect Dis. 2016;214(suppl 5):S471-S474. doi:10.1093/infdis/jiw274
3. Morrison TE. Reemergence of chikungunya virus. J Virol. 2014;88(20):11644-11647
Day(s) Performed
Tuesday, Thursday
Report Available
Same day/1 to 5 daysTest Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
87798
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| CHIKS | Chikungunya Virus, PCR, Serum | 81152-1 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 603833 | Chikungunya Virus, PCR, Serum | 81152-1 |
Testing Algorithm
For more information see Mosquito-borne Disease Laboratory Testing.
Special Instructions
Forms
If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.
mml-mosquitoborne