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Test ID: CHIKC Chikungunya Virus, PCR, Molecular Detection, Spinal Fluid

Additional Testing Requirements

Due to the short period in which chikungunya RNA may be detected in cerebrospinal fluid (CSF), testing serum for IgM- and IgG-class antibodies to chikungunya virus is also recommended. See CHIKV / Chikungunya IgM and IgG, Antibody, Serum.

Specimen Required

Supplies: Sarstedt 5 mL Aliquot Tube (T914)


Preferred: 12 x 75-mm screw cap vial

Acceptable: Sterile screw cap vial

Specimen Volume: 0.5 mL

Collection Instructions: Do not centrifuge.

Useful For

Qualitative detection of chikungunya virus in cerebrospinal fluid (CSF) after early symptom onset (ideally <7 days)


This test is not recommended for screening healthy patients.

Method Name

Real-Time Reverse Transcription Polymerase Chain Reaction (PCR)/DNA Probe Hybridization

Reporting Name

Chikungunya Virus, PCR, CSF

Specimen Type


Specimen Minimum Volume

0.3 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
CSF Refrigerated (preferred) 7 days
  Frozen  7 days

Clinical Information

Chikungunya virus (CHIK) is an RNA virus of the genus Alphavirus, family Togaviridae, and is transmitted mainly through the bite of infected mosquitoes in the genus Aedes (A aegypti and A albopictus). This is the same mosquito that transmits dengue, yellow fever, and Zika viruses. Most people infected with chikungunya virus will develop some symptoms, most commonly fever and joint pain. There is no specific antiviral treatment for chikungunya virus infection.


Most cases of disease have occurred in Africa, Asia, Europe, and the Indian and Pacific Oceans, but transmission of CHIK has been identified in Caribbean countries and South American regions, as well as foci in the southern United States. Infection with chikungunya virus may be suspected based on symptoms (fever, joint pain, and headache) and recent history of travel. A diagnosis of CHIK infection can be confirmed through laboratory tests on serum or cerebrospinal fluid.


This assay is designed to detect only species of clinical significance and is to be used for patients with a clinical history and symptoms consistent with chikungunya infection.

Reference Values



A positive test result indicates the presence of chikungunya virus RNA in the specimen.


A negative test result with a positive internal control indicates that chikungunya virus RNA is not detectable in the specimen.


A negative test result with a negative internal control is considered evidence of PCR inhibition or reagent failure. A new specimen should be collected for testing if clinically indicated.

Clinical Reference

1. Lanciotti RS, Kosoy OL, Laven JJ, Chikungunya virus in US travelers returning from India, 2006. Emerg Infect Dis 2007 May;13(5):764-767. Available at

2. Johnson BW, Russell BJ, Goodman CH: Laboratory Diagnosis of Chikungunya Virus Infections and Commercial Sources for Diagnostic Assays. J Infect Dis 2016 Dec 15;214(suppl 5):S471-S474. Available at

3. Morrison TE: Reemergence of chikungunya virus. J Virol 2014 Oct;88(20):11644-11647

Day(s) Performed

Tuesday, Thursday

Report Available

5 to 8 days

Test Classification

This test was developed, and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information


LOINC Code Information

Test ID Test Order Name Order LOINC Value
CHIKC Chikungunya Virus, PCR, CSF 81153-9


Result ID Test Result Name Result LOINC Value
603832 Chikungunya Virus, PCR, CSF 81153-9

Testing Algorithm

See Mosquito-borne Disease Laboratory Testing in Special Instructions.


If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.

Mayo Clinic Laboratories | Microbiology and Infectious Disease Catalog Additional Information: