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Test ID: BMIPB Borrelia miyamotoi Detection, PCR, Blood

Ordering Guidance

This assay does not detect the Borrelia species that cause Lyme disease. If Lyme disease is suspected, order SLYME / Lyme Antibody Modified 2-Tier with Reflex, Serum.

Specimen Required

Container/Tube: Lavender top (EDTA)

Specimen Volume: 1 mL

Collection Instructions: Send whole blood specimen in original tube. Do not aliquot.

Useful For

Aiding in the diagnosis of Borrelia miyamotoi infection in conjunction with clinical findings


Preferred method for detection of B miyamotoi using blood specimens

Testing Algorithm

For more information see Acute Tick-Borne Disease Testing Algorithm.

Method Name

Real-Time Polymerase Chain Reaction (PCR)

Reporting Name

Borrelia miyamotoi Detection, PCR, B

Specimen Type

Whole Blood EDTA

Specimen Minimum Volume

0.3 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Whole Blood EDTA Refrigerated 7 days

Clinical Information

Borrelia miyamotoi is a spirochetal bacterium, closely related to the Borrelia species that causes tick-borne relapsing fever (TBRF), and it is more distantly related to the Borrelia species that cause Lyme disease. This organism causes a febrile illness like TBRF, with body and join pain, fatigue, and, rarely, rash. B miyamotoi has been detected in Ixodes scapularis and Ixodes pacificus ticks. These ticks are also the vectors for Lyme disease, anaplasmosis, and babesiosis.


The preferred method for detecting B miyamotoi is real-time polymerase chain reaction. Less sensitive and specific methods for detecting B miyamotoi and agents of TBRF include serologic testing and identification of spirochetes in peripheral blood films or spinal fluid preparations. This assay does not detect the Borrelia species that cause Lyme disease.

Reference Values


Reference values apply to all ages.


A positive result indicates the presence of Borrelia miyamotoi DNA and is consistent with active or recent infection. While positive results are highly specific indicators of disease, they should be correlated with symptoms and clinical findings of tick-borne relapsing fever.

Clinical Reference

1. Gugliotta JL, Goethert HK, Berardi VP, Telford SR III: Meningoencephalitis from Borrelia miyamotoi in an immunocompromised patient. N Engl J Med. 2013 Jan 17;368(3):240-245

2. Fomenko NV, Borgoyakov VY, Panov VV: Genetic features of DNA of Borrelia miyamotoi transmitted by Ixodes persulcatus. Mol Genet Microbiol Virol 2011;26:60-65. doi: 10.3103/S0891416811020054

3. Platonov AE, Karan LS, Kolyasnikova NM, et al: Humans Infected with Relapsing Fever Spirochete Borrelia miyamotoi, Russia. Emerg Infect Dis. 2011 Oct;17(10):1816-1823

Day(s) Performed

Monday through Saturday

Report Available

Same day/1 to 4 days

Test Classification

This test was developed, and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information


LOINC Code Information

Test ID Test Order Name Order LOINC Value
BMIPB Borrelia miyamotoi Detection, PCR, B 82475-5


Result ID Test Result Name Result LOINC Value
618298 B. miyamotoi PCR, B 82475-5
Mayo Clinic Laboratories | Microbiology and Infectious Disease Catalog Additional Information: